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An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.

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Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice. However. current protocols for electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes which compromises embryo viability. Here. https://www.isbasvurusuyap.com/product-category/pull-through-knife-sharpeners/
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