Newcastle disease (ND) is a highly contagious viral disease caused by the paramyxovirus. which is a single-stranded ribonucleic acid (RNA) virus. This study was conducted to investigate ND outbreaks in 10 vaccinated or non-vaccinated broiler farms. collectively housing 9840 birds of various ages in the Chitwan and Nawalpur districts of Nepal from July to December 2021. https://www.lightemupsequences.com/