This study aims to investigate macroinvertebrate assemblage structure and composition across the three major waterbody types (temporary rivers. depression wetlands and semi-permanent dams) of the Eastern Cape Karoo. and to identify important environmental and spatial correlates of macroinvertebrate assemblage composition in the region. A total of 33 waterbodies (9 dams. https://www.roneverhart.com/Western-Digital-Ultrastar-DC-HC550-WUH721816ALE6L0-0F38460-16TB-7-2K-RPM-SATA-6Gb-s-512e-ISE-3-5in-Hard-Drive/